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melanoma cell line a375  (ATCC)


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    Structured Review

    ATCC melanoma cell line a375
    Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of <t>A375</t> cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.
    Melanoma Cell Line A375, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/melanoma+cell+line+a375/A-375/pmc13269842-52-0-13
    Average 99 stars, based on 5224 article reviews
    melanoma cell line a375 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4"

    Article Title: A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4

    Journal: Human Mutation

    doi: 10.1155/humu/4978880

    Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.
    Figure Legend Snippet: Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

    Techniques Used: Reverse Transcription, Biomarker Discovery, Knockdown, Migration, In Vitro

    Related Articles

    Cell Culture:

    Article Title: The Tumor Suppressor TPD52-Governed Endoplasmic Reticulum Stress is Modulated by APC Cdc20 .
    Article Snippet: .. Cell Culture and Transfection and Viral Infection: Human bladder cancer cell lines T24, 253J, J82, SW780, and 5637, melanoma cell line A375, and human embryonic kidney cell line HEK293T were obtained from ATCC and were cultured in a 5% CO2 incubator at 37 °C. ..

    Article Title: A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4
    Article Snippet: .. Melanoma cell line A375 (CRL‐1619) and human epidermal melanocytes (PCS‐200‐013) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia) and cultured as follows. .. The melanocytes were cultured in the specified growth kit (PCS‐200‐042, ATCC), and A375 cells were grown in DMEM (30‐2002, ATCC) added with 10% bovine calf serum (30‐2020, ATCC).

    Transfection:

    Article Title: The Tumor Suppressor TPD52-Governed Endoplasmic Reticulum Stress is Modulated by APC Cdc20 .
    Article Snippet: .. Cell Culture and Transfection and Viral Infection: Human bladder cancer cell lines T24, 253J, J82, SW780, and 5637, melanoma cell line A375, and human embryonic kidney cell line HEK293T were obtained from ATCC and were cultured in a 5% CO2 incubator at 37 °C. ..

    Infection:

    Article Title: The Tumor Suppressor TPD52-Governed Endoplasmic Reticulum Stress is Modulated by APC Cdc20 .
    Article Snippet: .. Cell Culture and Transfection and Viral Infection: Human bladder cancer cell lines T24, 253J, J82, SW780, and 5637, melanoma cell line A375, and human embryonic kidney cell line HEK293T were obtained from ATCC and were cultured in a 5% CO2 incubator at 37 °C. ..

    other:

    Article Title: Desmosome mutations impact the tumor microenvironment to promote melanoma proliferation
    Article Snippet: The melanoma cell line A375 was obtained from ATCC, authenticated using short tandem repeat profiling and tested for Mycoplasma contamination using a MycoAlert Mycoplasma Detection Kit (cat. no. LT07–318, Lonza).



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    In vitro cytotoxicity profiles of (A) rhein (RH), (B) rhein-bilosomes (BIL), and (C) rhein-limobilosomes (L-BIL) against <t>A375</t> melanoma cells and HDFa normal cell lines. The figure was generated by GraphPad Prism and retouched by Adobe Illustrator.
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    Image Search Results


    Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

    Journal: Human Mutation

    Article Title: A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4

    doi: 10.1155/humu/4978880

    Figure Lengend Snippet: Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

    Article Snippet: Melanoma cell line A375 (CRL‐1619) and human epidermal melanocytes (PCS‐200‐013) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia) and cultured as follows.

    Techniques: Reverse Transcription, Biomarker Discovery, Knockdown, Migration, In Vitro

    In vitro cytotoxicity profiles of (A) rhein (RH), (B) rhein-bilosomes (BIL), and (C) rhein-limobilosomes (L-BIL) against A375 melanoma cells and HDFa normal cell lines. The figure was generated by GraphPad Prism and retouched by Adobe Illustrator.

    Journal: Frontiers in Pharmacology

    Article Title: A dual edge-activation strategy for rhein bilosomes enhances intradermal delivery and anti-melanoma efficacy

    doi: 10.3389/fphar.2026.1798385

    Figure Lengend Snippet: In vitro cytotoxicity profiles of (A) rhein (RH), (B) rhein-bilosomes (BIL), and (C) rhein-limobilosomes (L-BIL) against A375 melanoma cells and HDFa normal cell lines. The figure was generated by GraphPad Prism and retouched by Adobe Illustrator.

    Article Snippet: For the cytotoxicity study, A375 human melanoma cell line was obtained from ATCC, Virginia, United States, while dimethyl sulfoxide was obtained from ThermoFisher Scientific, USA, and PBS was obtained from Biowest, USA.

    Techniques: In Vitro, Generated